Quick Overview for CHO HCP Lipase Panel (ABIN7670500)
适用
检测方法
应用范围
样品类型
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原理
- The detection of specific lipase proteins by Western blot, pertinent to the purification of antibody biologics from CHO cell platforms.
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特异性
- 8 antibodies specific for target lipases have been validated in CHO supernatant
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产品特性
- The majority of biological drugs are monoclonal antibodies and the majority of these are derived from CHO platforms. A major issue in the purification of antibodies from CHO is the persistence of lipase proteins through the bioprocess to the final drug product, with implications for product stability. HCP ELISAs measure total protein population with no resolution of individual protein identity, while mass spec is not suited to dynamic monitoring. This panel of antibodies is intended to be used for broad screening bioprocess samples for the presence of individual lipases that can then be subject to further, more specific testing, such as via an individual lipase ELISA.
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组件
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- anti-PLBD2 antibody (40 µL)
- anti-mLIPA antibody (20 µL)
- anti-PPT1 antibody (20 µL)
- anti-IAH1 antibody (20 µL)
- anti-PLD3 antibody (20 µL)
- anti-hLIPA antibody (20 µL)
- anti-PLA1A antibody (20 µL)
- anti-PLBL1 antibody (20 µL)
- Opal Pre-stained Protein Standard 10-245kDa (80 µL)
- Goat anti Rabbit IgG CY5 (100 µL)
- Goat anti Mouse IgG CY5 (50 µL)
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Tumor Necrosis Factor alpha (TNF alpha) ELISA Kit
TNF alpha 适用: 大鼠 Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Interleukin 1, beta (IL1B) ELISA KitIL1B 适用: 大鼠 Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Tumor Necrosis Factor alpha (TNF alpha) ELISA KitTNF alpha 适用: 小鼠 Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
High Mobility Group Box 1 (HMGB1) ELISA KitHMGB1 适用: 人 Colorimetric Sandwich ELISA 62.5 pg/mL - 4000 pg/mL Plasma, Serum
Amyloid beta 1-40 (Abeta 1-40) ELISA KitAbeta 1-40 适用: 人 Colorimetric Sandwich ELISA 7.813 pg/mL - 500 pg/mL Plasma, Serum, Tissue Homogenate
Amyloid beta 1-42 (Abeta 1-42) ELISA KitAbeta 1-42 适用: 人 Colorimetric Sandwich ELISA 4.688 pg/mL - 300 pg/mL Plasma, Serum, Tissue Homogenate
Lipopolysaccharides (LPS) ELISA Kit适用: Various Species Colorimetric Competition ELISA 12.35 ng/mL - 1000 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
High Mobility Group Box 1 (HMGB1) ELISA KitHMGB1 适用: 小鼠 Colorimetric Sandwich ELISA 46.88 pg/mL - 3000 pg/mL Plasma, Serum
Tumor Necrosis Factor alpha (TNF alpha) ELISA KitTNF alpha 适用: 人 AA 77-233 Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Plasma (EDTA), Plasma (citrate), Plasma (heparin), Serum
Interleukin 6 (IL6) ELISA KitIL6 适用: 人 Colorimetric Sandwich ELISA 7.8-500 pg/mL Cell Culture Supernatant, Plasma, Serum, Tissue Homogenate, Urine
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实验流程
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- Resolve CHO supernatant or lysate by SDS-PAGE and transfer to membrane for Western blot probe.
- Block membrane in Blocking Buffer for Fluorescent Western Blotting (e.g. ABIN925618) for 1 hour at room temperature with rocking.
- Probe by specific anti-lipase antibody using the entirety of the primary antibody aliquot provided into 10 mL of blocking buffer overnight at 4 °C with rocking.
- Wash membranes three times in PBS with 0.05 % Tween20
- Incubate secondary antibody 1:2000 (5 µL into 10 mL of blocking buffer) with anti-host species Cy5-conjugated secondary antibody, incubated for 1 hour with rocking at room temperature.
- Wash membranes three times in PBS with 0.05 % Tween20
- Image blot in fluorescent channel appropriate for Cy5 tag on secondary antibody and Cy2 channel to visualize Opal Pre-stained Protein Standard.
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试剂准备
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Prepare specific anti-lipase antibody using the entirety of the primary antibody aliquot provided into 10 mL of blocking buffer.
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限制
- 仅限研究用
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储存条件
- -20 °C
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